5KSW
DHODB-I74D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 79.990, 151.720, 214.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.921 - 2.470 |
| R-factor | 0.1793 |
| Rwork | 0.176 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ep1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.129 |
| Data reduction software | iMOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 2.300 | |
| Rmerge | 0.088 | 0.550 |
| Number of reflections | 47302 | |
| <I/σ(I)> | 12.2 | 2.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1 M sodium acetate pH 4.0, PEG 4,000 12% w/v, 3% v/v glycerol |
