5KS8
Crystal structure of two-subunit pyruvate carboxylase from Methylobacillus flagellatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 285.760, 285.760, 274.873 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.440 - 3.010 |
| R-factor | 0.22761 |
| Rwork | 0.225 |
| R-free | 0.27151 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | S. aureus pyruvate carboxylase |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.456 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Rmerge | 0.082 | 0.608 |
| Number of reflections | 81321 | |
| <I/σ(I)> | 11 | 1.8 |
| Completeness [%] | 95.7 | 96.2 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 19% (w/v) PEG3350, 2% tacsimate (pH 6.0) (Hampton), and 3% (v/v) ethanol |
