5KPD
Mouse pgp 34 linker deleted double EQ mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.953, 116.594, 375.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.416 - 3.350 |
R-factor | 0.2417 |
Rwork | 0.240 |
R-free | 0.28260 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5ko2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.208 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX (dev_2443) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.944 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.101 | 0.463 |
Number of reflections | 62273 | |
<I/σ(I)> | 17.5 | |
Completeness [%] | 96.5 | |
Redundancy | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | In the setup the rotein at 10.6 mg of 4 ul volume was diluted with 0.2 ul water and treated with 4 ul of 28% PEG400, 50 mM Glycine 9.0 and 100 mM NaCl. The reservoir solution conatined 100 mM Glycine 9.0pH, 150 mM NaCl, 10 mM NaN3 and 30% PEG400 |