5KOY
Mouse pgp 34 linker deleted bound with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.520, 116.439, 375.052 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.302 - 3.850 |
| R-factor | 0.2481 |
| Rwork | 0.246 |
| R-free | 0.28880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kpd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.724 | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 10.290 | 3.800 |
| Rmerge | 0.132 | 0.067 | 0.997 |
| Rmeas | 0.131 | ||
| Rpim | 0.035 | ||
| Total number of observations | 611650 | ||
| Number of reflections | 42251 | ||
| <I/σ(I)> | 6.1 | ||
| Completeness [%] | 99.9 | 99 | 99.5 |
| Redundancy | 14.5 | 15.9 | 9.5 |
| CC(1/2) | 0.998 | 0.372 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 100 mM glycine pH 9.0, 150 mM NaCl, 31% PEG400. After crystals grew, crystals were soaked in cryoprotectant solution with 18 mM ATP |
