5KNM
Human leukocyte antigen F (HLA-F) presents peptides and regulates immunity through interactions with NK-cell receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 231.820, 231.820, 73.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.410 - 3.300 |
| R-factor | 0.305 |
| Rwork | 0.303 |
| R-free | 0.32600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1psa |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.774 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.406 | 3.422 |
| High resolution limit [Å] | 3.304 | 3.304 |
| Rmerge | 0.141 | 1.136 |
| Number of reflections | 17859 | |
| <I/σ(I)> | 1.57 | 1.57 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 9.4 | 9.8 |
| CC(1/2) | 0.997 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 27% PEG 550 MME .1 M Tris-HCl 300 mM NaCl |
