5KNL
Crystal structure of S. pombe ubiquitin E1 (Uba1) in complex with Ubc15 and ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2014-07-31 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.111, 82.238, 135.376 |
| Unit cell angles | 102.10, 95.78, 90.86 |
Refinement procedure
| Resolution | 28.314 - 2.500 |
| R-factor | 0.2196 |
| Rwork | 0.216 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ii2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.699 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.051 | 0.289 |
| Number of reflections | 108289 | |
| <I/σ(I)> | 10.2 | 3.1 |
| Completeness [%] | 96.9 | 93.2 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.970 | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M Tris-HCl pH 8.0-8.5, 0.3-0.4 M lithium sulfate, 15-18% w/v polyethylene glycol 8000 |
