5KNC
Crystal structure of the 3 ADP-bound V1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 121.680, 126.490, 225.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.372 - 3.015 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.25320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5knb |
RMSD bond length | 0.003 |
RMSD bond angle | 0.660 |
Data scaling software | XDS |
Phasing software | PHASER (2.5.3) |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.015 | 3.020 |
Rmerge | 1.064 | |
Number of reflections | 67952 | |
<I/σ(I)> | 10.08 | 1.92 |
Completeness [%] | 99.2 | 95.4 |
Redundancy | 6.6 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | PEG3350, sodium fluoride, Bis-tris propane |