5KLI

Rhodobacter sphaeroides bc1 with stigmatellin and antimycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-21
Detector	MAR CCD 165 mm
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	118.861, 126.929, 127.891
Unit cell angles	64.64, 87.69, 61.80

Refinement procedure

Resolution	37.744 - 2.996
R-factor	0.2119
Rwork	0.211
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qjy
RMSD bond length	0.008
RMSD bond angle	1.093
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.070
High resolution limit [Å]	2.996	2.996
Rmerge		0.492
Rpim		0.464
Number of reflections	112899
<I/σ(I)>	6.04
Completeness [%]	92.7
Redundancy	2.5	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	9% PEG400, 10 mM Sr(NO3)2, 0.06% sucrose mono caprate, 200 mM histidine, 150 mM NaCl, stigmatellin, antimycin