5KL4

Wilms Tumor Protein (WT1) ZnF2-4 Q369H in complex with formylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-20
Detector	RAYONIX MX340-HS
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	35.766, 43.853, 54.808
Unit cell angles	81.95, 88.57, 88.51

Refinement procedure

Resolution	29.558 - 1.783
R-factor	0.218
Rwork	0.216
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r2r
RMSD bond length	0.015
RMSD bond angle	1.367
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2257: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.850
High resolution limit [Å]	1.783	1.783
Rmerge	0.073	0.540
Number of reflections	29040
<I/σ(I)>	10.7	1.7
Completeness [%]	92.9	69.6
Redundancy	3.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M CaCl2, 20% (w/v) PEG 3350