5KKP
Crystal Structure of Human Pseudouridylate Synthase 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-03 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.356, 73.400, 138.179 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.360 - 2.260 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.300 |
| High resolution limit [Å] | 2.260 | 6.130 | 2.260 |
| Rmerge | 0.101 | 0.074 | 0.823 |
| Rmeas | 0.105 | ||
| Rpim | 0.028 | ||
| Total number of observations | 364318 | ||
| Number of reflections | 27643 | ||
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 99.6 | 99.9 | 95.3 |
| Redundancy | 13.2 | 12.1 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 23% PEG 3350, 0.2 M Sodium formate, pH7.0 |
