5KIW
p97 ND1-L198W in complex with VIMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 153.772, 153.772, 240.641 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.268 - 3.410 |
| R-factor | 0.2362 |
| Rwork | 0.233 |
| R-free | 0.29394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ko8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.948 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.410 |
| Number of reflections | 22714 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 95.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 288 | 0.1 M Tris, pH 8, 15 % ethanol, 100 mM NaCl, 7 % MPD |
