5KFI

Human DNA polymerase eta-DNA ternary complex: reaction with 10 mM Mn2+ for 120s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.530, 98.530, 81.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.896 - 1.650
R-factor	0.1914
Rwork	0.190
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.896		1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.070	0.019	0.702
Rmeas	0.076	0.021	0.759
Total number of observations	355355
Number of reflections	53596	604	4020
<I/σ(I)>	19.96	77.18	2.65
Completeness [%]	98.7	94.7	100
CC(1/2)	0.999	0.999	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	298	17% PEG 2000MME, 0.1 M MES