5KE6

mouse Klf4 ZnF1-3 and TpG/CpA sequence DNA complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	50.870, 50.870, 130.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.678 - 1.990
R-factor	0.1997
Rwork	0.198
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m9e
RMSD bond length	0.008
RMSD bond angle	0.874
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2400: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.678	2.040
High resolution limit [Å]	1.990	1.990
Rmerge	0.073	0.982
Number of reflections	12435
<I/σ(I)>	16.58	2.32
Completeness [%]	99.9
Redundancy	7	7.2
CC(1/2)	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1M Tris-HCl pH8.5 , 0.25M NaCl and 20% polyethylene glycol 8000