5KDV
IMPa metallopeptidase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-09 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.280, 96.410, 100.920 |
Unit cell angles | 90.00, 124.66, 90.00 |
Refinement procedure
Resolution | 48.720 - 1.930 |
R-factor | 0.17314 |
Rwork | 0.172 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.530 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.720 | 2.030 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.088 | 0.489 |
Number of reflections | 90627 | |
<I/σ(I)> | 15 | 4.2 |
Completeness [%] | 98.8 | 97.9 |
Redundancy | 6.4 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | PEG 3350, (NH4)2SO4, Bicine pH 9.0 |