5KD4
Crystal Structure of Murine MHC-I H-2Dd in complex with Murine Beta2-Microglobulin and a Variant of Peptide (PVI10) of HIV gp120 MN Isolate (IGPGRAFYVI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0333 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.130, 90.300, 120.070 |
| Unit cell angles | 90.00, 113.19, 90.00 |
Refinement procedure
| Resolution | 47.088 - 3.050 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ecb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.167 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.189 | 0.950 |
| Number of reflections | 17628 | |
| <I/σ(I)> | 13 | 2.6 |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 7.6 | 7.6 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 15% PEG 20000, 0.1M Sodium Cacodylate |
