5KBX

Co-crystal structure of the Saccharomyces cerevisiae histidine phosphotransfer signaling protein Ypd1 and the receiver domain of its downstream response regulator Ssk1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97910, 0.97930, 0.9116
Spacegroup name	P 43 21 2
Unit cell lengths	71.696, 71.696, 176.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.270 - 2.800
R-factor	0.2325
Rwork	0.228
R-free	0.27850
Structure solution method	MAD
RMSD bond length	0.001
RMSD bond angle	0.350
Data reduction software	HKL-2000 (708)
Data scaling software	HKL-2000 (708)
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.700	2.900
High resolution limit [Å]	2.798	2.800
Rmerge	0.037	0.168
Number of reflections	10695	1163
<I/σ(I)>	34	9.2
Completeness [%]	89.0	44
Redundancy	1.9	1.8
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	296	0.2 M lithium sulfate, 0.1 M CAPS/NaOH pH 10.5, 1.2 M NaH2PO4/0.8 M K2HPO4