5K7U

Crystal structure of the catalytic domains of Mettl3/Mettl14 complex with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97934
Spacegroup name	P 41 21 2
Unit cell lengths	101.732, 101.732, 117.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.453 - 1.700
R-factor	0.1646
Rwork	0.163
R-free	0.18730
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.952
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	68473
<I/σ(I)>	38.9
Completeness [%]	100.0
Redundancy	16.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1M Tris pH 8.0, 20% PEG3350