5K1B

Crystal structure of the UAF1/USP12 complex in F222 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.283
Spacegroup name	F 2 2 2
Unit cell lengths	120.958, 158.929, 234.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 3.300
R-factor	0.23737
Rwork	0.235
R-free	0.28551
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.547
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	3.360
High resolution limit [Å]	3.300	3.300
Rmerge	0.102
Number of reflections	16052
<I/σ(I)>	28.2
Completeness [%]	98.1	96.4
Redundancy	8.4	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	0.1 M imidazole, 18% PEG 2000MME, 0.3 M ammonium citrate