5JYA
Structures of Streptococcus agalactiae GBS GAPDH in different enzymatic states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.025, 108.681, 90.986 |
| Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
| Resolution | 87.550 - 2.850 |
| R-factor | 0.1767 |
| Rwork | 0.174 |
| R-free | 0.21164 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4QX6_A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.333 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.550 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.057 | 0.241 |
| Number of reflections | 29605 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20-28% PEG4000, 0.1M Mes |
