5JY6

Structures of Streptococcus agalactiae GBS GAPDH in different enzymatic states

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	67.578, 107.465, 89.273
Unit cell angles	90.00, 104.07, 90.00

Refinement procedure

Resolution	86.600 - 2.000
R-factor	0.19597
Rwork	0.195
R-free	0.21625
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4QX6_A
RMSD bond length	0.008
RMSD bond angle	1.360
Data reduction software	XDS (March 30, 2013)
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.600	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.084	0.467
Number of reflections	82890
<I/σ(I)>	11.3	1.9
Completeness [%]	99.3	99.1
Redundancy	2.5	2.5
CC(1/2)	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20-28% PEG4000, 0.1M Mes