5JX0

Temperature sensitive D4 mutant L110F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-09-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	92.346, 102.916, 116.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.080 - 2.400
R-factor	0.22901
Rwork	0.228
R-free	0.25332
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4DOF_A
RMSD bond length	0.011
RMSD bond angle	1.476
Data reduction software	XDS (March 30, 2013)
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.080	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.054	0.658
Number of reflections	44146
<I/σ(I)>	14.8	1.3
Completeness [%]	99.9	100
Redundancy	5.4	5.4
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	8% PEG8000, 5% Glycerol, 0.1M Tris