5JVG
The large ribosomal subunit from Deinococcus radiodurans in complex with avilamycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 170.034, 412.632, 698.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.549 - 3.428 |
R-factor | 0.2135 |
Rwork | 0.211 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zjr |
RMSD bond length | 0.005 |
RMSD bond angle | 1.014 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.410 |
High resolution limit [Å] | 3.350 | 9.080 | 3.350 |
Rmerge | 0.168 | 0.052 | 0.886 |
Total number of observations | 1115549 | ||
Number of reflections | 290370 | ||
<I/σ(I)> | 4.7 | ||
Completeness [%] | 93.3 | 85.3 | 89.2 |
Redundancy | 3.8 | 4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293.15 | Ribosome solution containing 0.0065 mM (180 A/ml) of D50S in 10 mM Hepes pH=7.8, 15 mM MgCl2 and 75 mM NH4Cl crystallization buffer was mixed with 10 mM spermidine, 1 % ethanol and 0.5 % 2-ethyl-1,3-hexanediol precipitants. A 0.005 ml crystallization drop was hanged over 10 % ethanol and 5% 2-ethyl-1,3-hexanediol in ddw reservoir. |