5JV5

Trypanosome brucei Hypoxanthine-guanine phosphoribosyltranferase in complex with Guanosine 5' monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.95369
Spacegroup name	P 21 21 2
Unit cell lengths	93.944, 111.711, 45.226
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.011 - 2.730
R-factor	0.2399
Rwork	0.236
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jsq
RMSD bond length	0.002
RMSD bond angle	0.558
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASES
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	2.860
High resolution limit [Å]	2.730	2.730
Rmerge	0.160	0.760
Number of reflections	13262
<I/σ(I)>	8.6	1.9
Completeness [%]	99.9	99.2
Redundancy	7	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	25% PEG 3350, 0.2 M lithium sulfate and 0.1 M Bis-Tris