5JT8
Structural basis for the limited antibody cross reactivity between the mite allergens Blo t 1 and Der p 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-05-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.933 |
Spacegroup name | H 3 |
Unit cell lengths | 117.412, 117.412, 132.364 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.468 - 2.100 |
R-factor | 0.1728 |
Rwork | 0.170 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xkg |
RMSD bond length | 0.008 |
RMSD bond angle | 1.029 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.486 |
High resolution limit [Å] | 2.100 |
Number of reflections | 79280 |
<I/σ(I)> | 28.8 |
Completeness [%] | 100.0 |
Redundancy | 17.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 294 | single crystals were obtained with drops containing 4 ul sample solution containing 1 mg/ml rproBlo t 1, 0.5 mM YCl3, and 10 mM Tris-HCl pH 8.0, and 2 ul reservoir solution containing 4% w/v PEG-6000, 0.1 M Na-HEPES pH 6.6 and 0.8 M LiNO3 |