5JQ3
Crystal structure of Ebola glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.07193 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 114.258, 114.258, 307.377 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.190 - 2.230 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3csy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.244 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.190 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.204 | |
| Number of reflections | 38090 | |
| <I/σ(I)> | 17.4 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 57.8 | 15.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 9% (w/v) PEG 6000 and 0.1 M Sodium citrate tribasic dihydrate |
