5JP1

Structure of Xanthomonas campestris effector protein XopD bound to tomato SUMO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9282
Spacegroup name	P 64
Unit cell lengths	119.103, 119.103, 50.463
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.551 - 2.100
R-factor	0.1832
Rwork	0.181
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oiv 1tgz
RMSD bond length	0.002
RMSD bond angle	0.507
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.550	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.089	0.465
Number of reflections	23605
<I/σ(I)>	8.7	2
Completeness [%]	98.1	99.4
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M bicine (pH 9.0) 1.6M ammonium sulfate