5JNU

Crystal structure of mouse Low-Molecular Weight Protein Tyrosine Phosphatase type A (LMPTP-A) complexed with phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	37.384, 77.152, 92.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.576 - 2.535
R-factor	0.2103
Rwork	0.208
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jnr
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	Aimless (0.5.7)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.580	38.580	2.650
High resolution limit [Å]	2.530	8.780	2.530
Rmerge	0.138	0.053	0.600
Rmeas	0.157
Rpim	0.074
Total number of observations	40453
Number of reflections	9354
<I/σ(I)>	6.7
Completeness [%]	99.5	98.8	96.4
Redundancy	4.3	3.8	3.3
CC(1/2)	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	293	7% PEG 10K, Bis Tris, Ammonium Acetate; soaked in NaPO4 buffer before data collection