5JMO
X-ray structure of furin in complex with the inhibitory antibody Nb14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976300 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 169.775, 50.039, 144.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.381 - 1.998 |
| R-factor | 0.1647 |
| Rwork | 0.163 |
| R-free | 0.19710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4RYD (furin chain A or chain B) and 5JMR (antibody chain C or chain D) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.381 | 2.120 |
| High resolution limit [Å] | 1.998 | 1.998 |
| Number of reflections | 83341 | |
| <I/σ(I)> | 12.02 | |
| Completeness [%] | 98.8 | |
| Redundancy | 6.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | .1 M sodium acetate, pH 5.6, 16-18% PEG 3350 |
