5JL4
Inhibitor resistant mutant catalytic core domain of HIV-1 Integrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-13 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.192, 45.346, 49.040 |
| Unit cell angles | 63.96, 68.05, 62.43 |
Refinement procedure
| Resolution | 43.037 - 1.758 |
| R-factor | 0.1613 |
| Rwork | 0.159 |
| R-free | 0.19180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.945 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.037 |
| High resolution limit [Å] | 1.758 |
| Rmerge | 0.056 |
| Number of reflections | 29637 |
| <I/σ(I)> | 32.7 |
| Completeness [%] | 98.3 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.5 | .2M Ammonium Sulfate 0.1 M BIS-TRIS pH 5.5 25% w/v Polyethylene glycol 3,350 |
