5JKE
Crystal structure of human IZUMO1-JUNO complex (crystal form 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 64.182, 141.776, 144.861 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.300 - 2.860 |
| R-factor | 0.20429 |
| Rwork | 0.203 |
| R-free | 0.23817 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jk9 5jka |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.592 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.300 |
| High resolution limit [Å] | 2.860 |
| Number of reflections | 31399 |
| <I/σ(I)> | 28.5 |
| Completeness [%] | 100.0 |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG4000, 0.2 M Li2SO4, 0.1 M MES-NaOH pH 6.0 |
