5JK7
The X-ray structure of the DDB1-DCAF1-Vpr-UNG2 complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 119.900, 128.200, 129.200 |
| Unit cell angles | 75.11, 89.44, 65.37 |
Refinement procedure
| Resolution | 40.300 - 3.490 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.20650 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.300 |
| High resolution limit [Å] | 3.490 |
| Number of reflections | 77315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 289.15 | 100 mM Na Ctrate, pH 5.6, 11% PEG 20000 |
