5JK5
Phenylalanine hydroxylase from dictyostelium - BH2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.968, 85.326, 74.894 |
| Unit cell angles | 90.00, 110.23, 90.00 |
Refinement procedure
| Resolution | 37.445 - 2.071 |
| R-factor | 0.1677 |
| Rwork | 0.165 |
| R-free | 0.22760 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.070 |
| Rmerge | 0.040 |
| Number of reflections | 50582 |
| <I/σ(I)> | 9.3 |
| Completeness [%] | 99.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 26%(w/v) PEG 2000, 0.06 M PIPES |
