5JJI
Rho transcription termination factor bound to rU7 and 6 ADP-BeF3 molecules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.997, 198.567, 111.448 |
| Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
| Resolution | 47.425 - 2.601 |
| R-factor | 0.2202 |
| Rwork | 0.219 |
| R-free | 0.24630 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.575 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.425 | 2.690 |
| High resolution limit [Å] | 2.601 | 2.601 |
| Rmerge | 0.081 | 0.630 |
| Number of reflections | 88380 | |
| <I/σ(I)> | 14 | 1.8 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | 200 mM KOAc, 40% MPD, 0.5% ethyl acetate |
