5JIY
Structure of G9a SET-domain with Histone H3K9norLeucine mutant peptide and bound S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 178 |
| Detector technology | CCD |
| Collection date | 2013-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0010 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.611, 78.463, 73.084 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 38.950 - 1.480 |
| R-factor | 0.2105 |
| Rwork | 0.209 |
| R-free | 0.23345 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o8j |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.967 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.950 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.725 | |
| Number of reflections | 100580 | |
| <I/σ(I)> | 14.15 | 2 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M Tri-ammonium citrate pH 7 and 20% w/v polyethylene glycol 3350 |
