5JII

Crystal structure of human IgG1-Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	49.384, 75.088, 149.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.889 - 1.790
R-factor	0.1828
Rwork	0.181
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oqo
RMSD bond length	0.013
RMSD bond angle	1.403
Data reduction software	XDS (VERSION January 10, 2014)
Data scaling software	XDS
Phasing software	PHENIX (1.10-2247)
Refinement software	PHENIX (dev_1894)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.890	1.854
High resolution limit [Å]	1.790	1.790
Rmerge	0.047	0.930
Number of reflections	52192
<I/σ(I)>	15.87	1.52
Completeness [%]	97.8	95.97
Redundancy	4.7	4.6
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.05 M Sodium phosphate, 16% (w/v) PEG 3350