5JI1

Crystal Structure of GDF8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	29.606, 77.739, 119.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.965 - 2.250
R-factor	0.2377
Rwork	0.235
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hh2
RMSD bond length	0.003
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	Aimless (0.5.23)
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.380	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.079	1.102
Rmeas	0.085
Rpim	0.032
Total number of observations	98443
Number of reflections	13830
<I/σ(I)>	18.2
Completeness [%]	100.0	100
Redundancy	7.1	7.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100mM 2-(N-morpholino)ethanesulfonic acid pH 6.0, 40% 2-Methyl-2,4-pentanediol