5JHH
Crystal structure of the ternary complex between the human RhoA, its inhibitor and the DH/PH domain of human ARHGEF11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-15 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.541782 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.855, 119.049, 90.526 |
| Unit cell angles | 90.00, 114.39, 90.00 |
Refinement procedure
| Resolution | 82.440 - 2.300 |
| R-factor | 0.1955 |
| Rwork | 0.193 |
| R-free | 0.23904 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.619 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.440 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 | 0.513 |
| Number of reflections | 73383 | |
| <I/σ(I)> | 19.268 | 2.7 |
| Completeness [%] | 96.3 | 93.2 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 2% v/v Tacsimate pH 5.0, 0.1M Sodium citrate tribasic dihydrate pH 5.6, 16% PEG 3350 |
