5JH5

Structural Basis for the Hierarchical Assembly of the Core of PRC1.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-03
Detector	NOIR-1
Wavelength(s)	0.979620
Spacegroup name	P 21 21 21
Unit cell lengths	70.049, 73.796, 123.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.282 - 2.550
R-factor	0.211
Rwork	0.206
R-free	0.25730
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.670
Data reduction software	XDS
Data scaling software	XDS
Phasing software	autoSHARP
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.282	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.114	0.970
Number of reflections	21463
<I/σ(I)>	15.5	2
Completeness [%]	99.9	99.5
Redundancy	7	6.1
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25	298	100 mM HEPES, 10 % 2-methyl-2,4-pentanediol, 10 mM NaCl