5JGD
Crystal structure of human TAK1/TAB1 fusion protein in complex with ligand 12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-12-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.167, 133.673, 141.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.040 - 3.101 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eva |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.237 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.040 | 70.555 | 3.270 |
| High resolution limit [Å] | 3.101 | 9.810 | 3.101 |
| Rmerge | 0.042 | 0.498 | |
| Number of reflections | 10345 | ||
| <I/σ(I)> | 14.1 | 14.9 | 1.4 |
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 7.1 | 5.8 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 1.7M sodium potassium phosphate, 20%(v/v) Glycerol as cryoprotectant |
