5JER

Structure of Rotavirus NSP1 bound to IRF-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	120
Detector technology	CCD
Collection date	2015-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	67.772, 107.924, 135.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.940 - 2.913
R-factor	0.2133
Rwork	0.210
R-free	0.25030
RMSD bond length	0.002
RMSD bond angle	0.589
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.940
High resolution limit [Å]	2.900
Number of reflections	22409
<I/σ(I)>	15.7
Completeness [%]	100.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-Tris pH 5.5, 200 mM MgCl2, 23% PEG 3350