5JEO

Phosphorylated Rotavirus NSP1 in complex with IRF-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	120
Detector technology	CCD
Collection date	2015-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	72.982, 72.982, 127.551
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.893 - 1.719
R-factor	0.1698
Rwork	0.169
R-free	0.18320
RMSD bond length	0.007
RMSD bond angle	1.005
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.893	1.750
High resolution limit [Å]	1.719	1.720
Rmerge	0.068
Number of reflections	42593
<I/σ(I)>	44.5	1.9
Completeness [%]	100.0	100
Redundancy	11.8	10.9
CC(1/2)	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M HEPES sodium pH7.5, 0.8 M NaH2PO4, 0.8 M KH2PO4