5JEK
Phosphorylated MAVS in complex with IRF-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2015-05-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.800, 104.910, 66.270 |
| Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
| Resolution | 63.661 - 2.400 |
| R-factor | 0.1945 |
| Rwork | 0.189 |
| R-free | 0.25060 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.605 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 64.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 19995 |
| <I/σ(I)> | 14.3 |
| Completeness [%] | 100.0 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-Tris pH 5.5, 80 mM MgCl2, 20% PEG 3350 |
