5JEJ

Phosphorylated STING in complex with IRF-3 CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	120
Detector technology	CCD
Collection date	2015-04-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 1 2 1
Unit cell lengths	64.076, 56.401, 75.548
Unit cell angles	90.00, 104.64, 90.00

Refinement procedure

Resolution	44.654 - 2.000
R-factor	0.1797
Rwork	0.176
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.004
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.654
High resolution limit [Å]	2.000
Number of reflections	35085
<I/σ(I)>	14
Completeness [%]	98.2
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-Tris, pH 5.5, 200 mM MgCl2, 20% PEG 3350