5JBQ

EF-TU (ESCHERICHIA COLI) IN COMPLEX WITH THIOMURACIN ANALOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-30
Detector	RIGAKU SATURN 92
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	80.350, 125.340, 45.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.690 - 2.006
R-factor	0.2069
Rwork	0.204
R-free	0.25270
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	BUSTER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.690	2.012
High resolution limit [Å]	2.006	2.006
Rmerge	0.065	0.250
Number of reflections	31079
<I/σ(I)>	15.7	4.2
Completeness [%]	98.5	66.2
Redundancy	4.7	2.6
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	0.2M MgSO4, 18% PEG3350, 100mM Tris Cryo 20% Ethylene Glycol