5JB2
Crystal structure of chicken LGP2 with 5'ppp 10-mer dsRNA and ADP-AlF4-Mg2+ at 2.2 A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.140, 96.580, 122.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.930 - 2.200 |
R-factor | 0.1909 |
Rwork | 0.188 |
R-free | 0.24457 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jaj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.365 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.930 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.169 | 0.835 |
Number of reflections | 43057 | |
<I/σ(I)> | 8.73 | 1.87 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 6.64 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | chLGP2, directly after size exclusion chromatography, was mixed with dsRNA in a 1:1 ratio and incubated for 30 minutes on ice. The complex was concentrated with an Amicon Ultra concentrator to around 10 mg/ml and then 2 mM ADP:AlF4 and 2 mM MgCl2 were added. 5 prime triphosphate 10-mer dsRNA:ADP:AlF4 complex was mixed with reservoir buffer (0.1 M Mg formate, 20% PEG3350M) in a 2:1 ratio. |