5JA4
Crystal structure of human TONSL and MCM2 HBDs binding to a histone H3-H4 tetramer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 139.506, 139.506, 72.896 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.513 - 2.424 |
R-factor | 0.2033 |
Rwork | 0.201 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bnv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.316 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1760) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.400 | 2.430 |
Number of reflections | 31146 | |
<I/σ(I)> | 23.1 | 1.8 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.1M MES pH 5.6, 7% isopropanol |