5J7C

A picomolar affinity FN3 domain in complex with hen egg-white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	54.858, 87.725, 100.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.510 - 2.535
R-factor	0.2192
Rwork	0.218
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fnf 4z98
RMSD bond length	0.002
RMSD bond angle	0.562
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155:000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.510	2.626
High resolution limit [Å]	2.535	2.535
Rmerge	0.244	1.264
Number of reflections	16719
<I/σ(I)>	8.9	2.13
Completeness [%]	99.0	96
Redundancy	6.7	6.5
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	10% PEG 6000, 0.1 M Bicine, pH 8.8