5J6X

Crystal structure of the apo-Zalpha of Zebrafish PKZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-27
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 41
Unit cell lengths	44.304, 44.304, 71.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.686 - 2.493
R-factor	0.1915
Rwork	0.182
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kmf
RMSD bond length	0.009
RMSD bond angle	1.107
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.690
High resolution limit [Å]	2.490
Rmerge	0.152
Number of reflections	4804
<I/σ(I)>	35.98
Completeness [%]	98.9
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	277.15	High concentration (3mM) of the purified protein in 20 mM HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid], 80 mM Sodium chloride was stored at 4degC. Crystals were cryo-protected with 35% (v/v) PEG400.