5J6H

Recognition of the MHC class Ib molecule H2-Q10 by the natural killer cell receptor Ly49C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9436
Spacegroup name	P 21 21 21
Unit cell lengths	55.420, 81.660, 97.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.470 - 2.300
R-factor	0.1802
Rwork	0.177
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yn6
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.000
High resolution limit [Å]	2.300
Rmerge	0.241
Number of reflections	20349
<I/σ(I)>	6.6
Completeness [%]	99.8
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	8% Peg3350, 0.1M sodium malonate ph 5.0, 0.01M b-nicotinamide adenine dinucleotide hydrate