5J5Y

Translation initiation factor 4E in complex with m2(7,2'O)GppCCl2ppG mRNA 5' cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 1
Unit cell lengths	38.040, 38.060, 146.740
Unit cell angles	88.36, 95.63, 103.54

Refinement procedure

Resolution	37.001 - 1.750
R-factor	0.239
Rwork	0.236
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.007
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.750	1.750
Rmerge	0.052	0.430
Number of reflections	72989
<I/σ(I)>	12.26
Completeness [%]	90.6
Redundancy	2.66
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	15% PEG 3350, 2% 1,4-dioxane, 0.1 M Tris